anti-gr1 antibody clone 1a8 Search Results


rat anti-gr-1 antibody (ly6g  (Becton Dickinson)
90
Becton Dickinson rat anti-gr-1 antibody (ly6g
MMP-8 decreases neutrophil infiltration. A: Mean ± SEM of results of gene expression analysis of chemokines (IL-1β) and CXCL1 mRNA transcripts in whole cornea of animals subjected to ocular burn with desiccating stress for 2 or 5 days and topically treated with either Dex or BSS in MMP-8KO or WT mice. ***P < 0.001; ****P < 0.0001 BSS versus UT in WT, BSS versus UT in MMP-8KO. B: Representative merged pictures of IL-1β (green) immunofluorescent staining of central cornea cryosections from animals subjected to a combined model of alkali burn and dry eye topically treated with either Dex or BSS in wild-type (WT) or MMP-8KO mice. Counterstaining was PI = red. C: Representative pictures of <t>Gr-1+</t> cells (red) of central cornea cryosections from animals subjected to a combined model of alkali burn and dry eye topically treated with either Dex or BSS in wild-type (WT) or MMP-8KO mice (10×). Insets are high magnification of the area below. D: Bar graphs (mean ± SEM) of Gr-1+ cell counts in whole cornea/groups. E: Myeloperoxidase (MPO) activity in whole corneas lysates from corneas subjected to ocular burn with concomitant desiccating stress and topically treated with either Dex or BSS in wild-type (WT) or MMP-8KO mice (mean ± SEM). Dex, Dexamethasone Sodium Phosphate; BSS, Balanced Salt Solution; UT= untreated control; NC=negative control
Rat Anti Gr 1 Antibody (Ly6g, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti-gr1+antibody+clone+1a8/ly6g+antibody/pmc04946998-71-7-14
Average 90 stars, based on 1 article reviews
rat anti-gr-1 antibody (ly6g - by Bioz Stars, 2026-10
90/100 stars
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anti-gr1 (1a8  (Becton Dickinson)
90
Becton Dickinson anti-gr1 (1a8
MMP-8 decreases neutrophil infiltration. A: Mean ± SEM of results of gene expression analysis of chemokines (IL-1β) and CXCL1 mRNA transcripts in whole cornea of animals subjected to ocular burn with desiccating stress for 2 or 5 days and topically treated with either Dex or BSS in MMP-8KO or WT mice. ***P < 0.001; ****P < 0.0001 BSS versus UT in WT, BSS versus UT in MMP-8KO. B: Representative merged pictures of IL-1β (green) immunofluorescent staining of central cornea cryosections from animals subjected to a combined model of alkali burn and dry eye topically treated with either Dex or BSS in wild-type (WT) or MMP-8KO mice. Counterstaining was PI = red. C: Representative pictures of <t>Gr-1+</t> cells (red) of central cornea cryosections from animals subjected to a combined model of alkali burn and dry eye topically treated with either Dex or BSS in wild-type (WT) or MMP-8KO mice (10×). Insets are high magnification of the area below. D: Bar graphs (mean ± SEM) of Gr-1+ cell counts in whole cornea/groups. E: Myeloperoxidase (MPO) activity in whole corneas lysates from corneas subjected to ocular burn with concomitant desiccating stress and topically treated with either Dex or BSS in wild-type (WT) or MMP-8KO mice (mean ± SEM). Dex, Dexamethasone Sodium Phosphate; BSS, Balanced Salt Solution; UT= untreated control; NC=negative control
Anti Gr1 (1a8, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti-gr1+antibody+clone+1a8/fitc/pmc05539354-342-17-30
Average 90 stars, based on 1 article reviews
anti-gr1 (1a8 - by Bioz Stars, 2026-10
90/100 stars
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ly6g  (Boster Bio)
94
Boster Bio ly6g
MMP-8 decreases neutrophil infiltration. A: Mean ± SEM of results of gene expression analysis of chemokines (IL-1β) and CXCL1 mRNA transcripts in whole cornea of animals subjected to ocular burn with desiccating stress for 2 or 5 days and topically treated with either Dex or BSS in MMP-8KO or WT mice. ***P < 0.001; ****P < 0.0001 BSS versus UT in WT, BSS versus UT in MMP-8KO. B: Representative merged pictures of IL-1β (green) immunofluorescent staining of central cornea cryosections from animals subjected to a combined model of alkali burn and dry eye topically treated with either Dex or BSS in wild-type (WT) or MMP-8KO mice. Counterstaining was PI = red. C: Representative pictures of <t>Gr-1+</t> cells (red) of central cornea cryosections from animals subjected to a combined model of alkali burn and dry eye topically treated with either Dex or BSS in wild-type (WT) or MMP-8KO mice (10×). Insets are high magnification of the area below. D: Bar graphs (mean ± SEM) of Gr-1+ cell counts in whole cornea/groups. E: Myeloperoxidase (MPO) activity in whole corneas lysates from corneas subjected to ocular burn with concomitant desiccating stress and topically treated with either Dex or BSS in wild-type (WT) or MMP-8KO mice (mean ± SEM). Dex, Dexamethasone Sodium Phosphate; BSS, Balanced Salt Solution; UT= untreated control; NC=negative control
Ly6g, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti-gr1+antibody+clone+1a8/Anti-Ms+Ly6G+Purified+Low+Endotoxin+Monoclonal+Antibody/pm33889520-466-15-46
Average 94 stars, based on 1 article reviews
ly6g - by Bioz Stars, 2026-10
94/100 stars
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gr1 1a8  (Becton Dickinson)
90
Becton Dickinson gr1 1a8
MMP-8 decreases neutrophil infiltration. A: Mean ± SEM of results of gene expression analysis of chemokines (IL-1β) and CXCL1 mRNA transcripts in whole cornea of animals subjected to ocular burn with desiccating stress for 2 or 5 days and topically treated with either Dex or BSS in MMP-8KO or WT mice. ***P < 0.001; ****P < 0.0001 BSS versus UT in WT, BSS versus UT in MMP-8KO. B: Representative merged pictures of IL-1β (green) immunofluorescent staining of central cornea cryosections from animals subjected to a combined model of alkali burn and dry eye topically treated with either Dex or BSS in wild-type (WT) or MMP-8KO mice. Counterstaining was PI = red. C: Representative pictures of <t>Gr-1+</t> cells (red) of central cornea cryosections from animals subjected to a combined model of alkali burn and dry eye topically treated with either Dex or BSS in wild-type (WT) or MMP-8KO mice (10×). Insets are high magnification of the area below. D: Bar graphs (mean ± SEM) of Gr-1+ cell counts in whole cornea/groups. E: Myeloperoxidase (MPO) activity in whole corneas lysates from corneas subjected to ocular burn with concomitant desiccating stress and topically treated with either Dex or BSS in wild-type (WT) or MMP-8KO mice (mean ± SEM). Dex, Dexamethasone Sodium Phosphate; BSS, Balanced Salt Solution; UT= untreated control; NC=negative control
Gr1 1a8, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti-gr1+antibody+clone+1a8/gr1+1a8+antibody/10__1128_slash_iai__00656___09-102-14-17
Average 90 stars, based on 1 article reviews
gr1 1a8 - by Bioz Stars, 2026-10
90/100 stars
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pe-labeled anti-gr-1 1a8  (Becton Dickinson)
90
Becton Dickinson pe-labeled anti-gr-1 1a8
MMP-8 decreases neutrophil infiltration. A: Mean ± SEM of results of gene expression analysis of chemokines (IL-1β) and CXCL1 mRNA transcripts in whole cornea of animals subjected to ocular burn with desiccating stress for 2 or 5 days and topically treated with either Dex or BSS in MMP-8KO or WT mice. ***P < 0.001; ****P < 0.0001 BSS versus UT in WT, BSS versus UT in MMP-8KO. B: Representative merged pictures of IL-1β (green) immunofluorescent staining of central cornea cryosections from animals subjected to a combined model of alkali burn and dry eye topically treated with either Dex or BSS in wild-type (WT) or MMP-8KO mice. Counterstaining was PI = red. C: Representative pictures of <t>Gr-1+</t> cells (red) of central cornea cryosections from animals subjected to a combined model of alkali burn and dry eye topically treated with either Dex or BSS in wild-type (WT) or MMP-8KO mice (10×). Insets are high magnification of the area below. D: Bar graphs (mean ± SEM) of Gr-1+ cell counts in whole cornea/groups. E: Myeloperoxidase (MPO) activity in whole corneas lysates from corneas subjected to ocular burn with concomitant desiccating stress and topically treated with either Dex or BSS in wild-type (WT) or MMP-8KO mice (mean ± SEM). Dex, Dexamethasone Sodium Phosphate; BSS, Balanced Salt Solution; UT= untreated control; NC=negative control
Pe Labeled Anti Gr 1 1a8, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti-gr1+antibody+clone+1a8/pe+labeled+anti+gr+1+1a8/pmc03916398-263-53-56
Average 90 stars, based on 1 article reviews
pe-labeled anti-gr-1 1a8 - by Bioz Stars, 2026-10
90/100 stars
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anti-gr1:bb700  (Becton Dickinson)
90
Becton Dickinson anti-gr1:bb700
MMP-8 decreases neutrophil infiltration. A: Mean ± SEM of results of gene expression analysis of chemokines (IL-1β) and CXCL1 mRNA transcripts in whole cornea of animals subjected to ocular burn with desiccating stress for 2 or 5 days and topically treated with either Dex or BSS in MMP-8KO or WT mice. ***P < 0.001; ****P < 0.0001 BSS versus UT in WT, BSS versus UT in MMP-8KO. B: Representative merged pictures of IL-1β (green) immunofluorescent staining of central cornea cryosections from animals subjected to a combined model of alkali burn and dry eye topically treated with either Dex or BSS in wild-type (WT) or MMP-8KO mice. Counterstaining was PI = red. C: Representative pictures of <t>Gr-1+</t> cells (red) of central cornea cryosections from animals subjected to a combined model of alkali burn and dry eye topically treated with either Dex or BSS in wild-type (WT) or MMP-8KO mice (10×). Insets are high magnification of the area below. D: Bar graphs (mean ± SEM) of Gr-1+ cell counts in whole cornea/groups. E: Myeloperoxidase (MPO) activity in whole corneas lysates from corneas subjected to ocular burn with concomitant desiccating stress and topically treated with either Dex or BSS in wild-type (WT) or MMP-8KO mice (mean ± SEM). Dex, Dexamethasone Sodium Phosphate; BSS, Balanced Salt Solution; UT= untreated control; NC=negative control
Anti Gr1:Bb700, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti-gr1+antibody+clone+1a8/bb515/pmc06581587-156-13-15
Average 90 stars, based on 1 article reviews
anti-gr1:bb700 - by Bioz Stars, 2026-10
90/100 stars
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rat anti gr 1  (R&D Systems)
94
R&D Systems rat anti gr 1
MMP-8 decreases neutrophil infiltration. A: Mean ± SEM of results of gene expression analysis of chemokines (IL-1β) and CXCL1 mRNA transcripts in whole cornea of animals subjected to ocular burn with desiccating stress for 2 or 5 days and topically treated with either Dex or BSS in MMP-8KO or WT mice. ***P < 0.001; ****P < 0.0001 BSS versus UT in WT, BSS versus UT in MMP-8KO. B: Representative merged pictures of IL-1β (green) immunofluorescent staining of central cornea cryosections from animals subjected to a combined model of alkali burn and dry eye topically treated with either Dex or BSS in wild-type (WT) or MMP-8KO mice. Counterstaining was PI = red. C: Representative pictures of <t>Gr-1+</t> cells (red) of central cornea cryosections from animals subjected to a combined model of alkali burn and dry eye topically treated with either Dex or BSS in wild-type (WT) or MMP-8KO mice (10×). Insets are high magnification of the area below. D: Bar graphs (mean ± SEM) of Gr-1+ cell counts in whole cornea/groups. E: Myeloperoxidase (MPO) activity in whole corneas lysates from corneas subjected to ocular burn with concomitant desiccating stress and topically treated with either Dex or BSS in wild-type (WT) or MMP-8KO mice (mean ± SEM). Dex, Dexamethasone Sodium Phosphate; BSS, Balanced Salt Solution; UT= untreated control; NC=negative control
Rat Anti Gr 1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti-gr1+antibody+clone+1a8/Mouse+Ly-6G+(Gr-1)+Antibody/pmc04956003-54-16-19
Average 94 stars, based on 1 article reviews
rat anti gr 1 - by Bioz Stars, 2026-10
94/100 stars
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unconjugated anti gr 1 antibody  (Bio X Cell)
96
Bio X Cell unconjugated anti gr 1 antibody
MMP-8 decreases neutrophil infiltration. A: Mean ± SEM of results of gene expression analysis of chemokines (IL-1β) and CXCL1 mRNA transcripts in whole cornea of animals subjected to ocular burn with desiccating stress for 2 or 5 days and topically treated with either Dex or BSS in MMP-8KO or WT mice. ***P < 0.001; ****P < 0.0001 BSS versus UT in WT, BSS versus UT in MMP-8KO. B: Representative merged pictures of IL-1β (green) immunofluorescent staining of central cornea cryosections from animals subjected to a combined model of alkali burn and dry eye topically treated with either Dex or BSS in wild-type (WT) or MMP-8KO mice. Counterstaining was PI = red. C: Representative pictures of <t>Gr-1+</t> cells (red) of central cornea cryosections from animals subjected to a combined model of alkali burn and dry eye topically treated with either Dex or BSS in wild-type (WT) or MMP-8KO mice (10×). Insets are high magnification of the area below. D: Bar graphs (mean ± SEM) of Gr-1+ cell counts in whole cornea/groups. E: Myeloperoxidase (MPO) activity in whole corneas lysates from corneas subjected to ocular burn with concomitant desiccating stress and topically treated with either Dex or BSS in wild-type (WT) or MMP-8KO mice (mean ± SEM). Dex, Dexamethasone Sodium Phosphate; BSS, Balanced Salt Solution; UT= untreated control; NC=negative control
Unconjugated Anti Gr 1 Antibody, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti-gr1+antibody+clone+1a8/InVivoMAb+anti-mouse+Ly6G+Ly6C/pmc04389265-146-10-14
Average 96 stars, based on 1 article reviews
unconjugated anti gr 1 antibody - by Bioz Stars, 2026-10
96/100 stars
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anti ly6g gr1 antibody  (Bio X Cell)
95
Bio X Cell anti ly6g gr1 antibody
Different <t>Ly6G+</t> myeloid cells in the wound healing oral barrier tissue of ZOL mice. A, immunohistology with anti-Ly6G antibody demonstrated clusters of Ly6G+ cells at the epithelial wound margin (not shown) and at the connective tissue interfacing palatal bone (B), both of which were adjacent to the tooth extraction site on day 3. In ZOL mice, fewer Ly6G+ cells were present in both cluster areas. At week 2, ZOL mice exhibited more Ly6G+ cells, in particular adjacent to jawbone oral exposure and/or pustule/fistula (P/F) areas. In some specimens, Ly6G+ cells (arrows) were found around osteoclast (Oc) and in necrotic bone (B). B, the number of Ly6G+ cells in the oral barrier tissue excluding the area of obvious jawbone exposure and P/F areas. *, p < 0.05; n = 4 in each group. C, flow cytometric analysis of the dissociated cells from oral barrier tissue harvested at week 2 of tooth extraction showed a large variation in size and granularity as shown in forward and side scatters. The back gating of CD11b+GR1high cells revealed the significant reduction of these cells in ZOL mice. D, dense nucleus small cells (arrowheads) were characteristically associated with TRAP+ osteoclasts (Oc) and necrotic alveolar bone (Nec) in ZOL mice at week 2 of tooth extraction but not found in control (NaCl) mice. Ly6G+ cells in control (NaCl) mice were PMN cells (arrows), whereas Ly6G+ cells in ZOL mice included small cells that did not exhibit a typical PMN cell structure (arrowheads).
Anti Ly6g Gr1 Antibody, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti-gr1+antibody+clone+1a8/InVivoPlus+anti-mouse+Ly6G/pmc05034053-920-16-23
Average 95 stars, based on 1 article reviews
anti ly6g gr1 antibody - by Bioz Stars, 2026-10
95/100 stars
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anti ly 6g pe  (ProSci Incorporated)
91
ProSci Incorporated anti ly 6g pe
Different <t>Ly6G+</t> myeloid cells in the wound healing oral barrier tissue of ZOL mice. A, immunohistology with anti-Ly6G antibody demonstrated clusters of Ly6G+ cells at the epithelial wound margin (not shown) and at the connective tissue interfacing palatal bone (B), both of which were adjacent to the tooth extraction site on day 3. In ZOL mice, fewer Ly6G+ cells were present in both cluster areas. At week 2, ZOL mice exhibited more Ly6G+ cells, in particular adjacent to jawbone oral exposure and/or pustule/fistula (P/F) areas. In some specimens, Ly6G+ cells (arrows) were found around osteoclast (Oc) and in necrotic bone (B). B, the number of Ly6G+ cells in the oral barrier tissue excluding the area of obvious jawbone exposure and P/F areas. *, p < 0.05; n = 4 in each group. C, flow cytometric analysis of the dissociated cells from oral barrier tissue harvested at week 2 of tooth extraction showed a large variation in size and granularity as shown in forward and side scatters. The back gating of CD11b+GR1high cells revealed the significant reduction of these cells in ZOL mice. D, dense nucleus small cells (arrowheads) were characteristically associated with TRAP+ osteoclasts (Oc) and necrotic alveolar bone (Nec) in ZOL mice at week 2 of tooth extraction but not found in control (NaCl) mice. Ly6G+ cells in control (NaCl) mice were PMN cells (arrows), whereas Ly6G+ cells in ZOL mice included small cells that did not exhibit a typical PMN cell structure (arrowheads).
Anti Ly 6g Pe, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti-gr1+antibody+clone+1a8/Ly6G%2FLy6C+Antibody+%5BRB6-8C5%5D%2C+Rat+IgG2a/pm32532837-69-15-20
Average 91 stars, based on 1 article reviews
anti ly 6g pe - by Bioz Stars, 2026-10
91/100 stars
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phycoerythrinindotricarbocyanine–anti-gr1 (rb6-8c5)  (Becton Dickinson)
90
Becton Dickinson phycoerythrinindotricarbocyanine–anti-gr1 (rb6-8c5)
Different <t>Ly6G+</t> myeloid cells in the wound healing oral barrier tissue of ZOL mice. A, immunohistology with anti-Ly6G antibody demonstrated clusters of Ly6G+ cells at the epithelial wound margin (not shown) and at the connective tissue interfacing palatal bone (B), both of which were adjacent to the tooth extraction site on day 3. In ZOL mice, fewer Ly6G+ cells were present in both cluster areas. At week 2, ZOL mice exhibited more Ly6G+ cells, in particular adjacent to jawbone oral exposure and/or pustule/fistula (P/F) areas. In some specimens, Ly6G+ cells (arrows) were found around osteoclast (Oc) and in necrotic bone (B). B, the number of Ly6G+ cells in the oral barrier tissue excluding the area of obvious jawbone exposure and P/F areas. *, p < 0.05; n = 4 in each group. C, flow cytometric analysis of the dissociated cells from oral barrier tissue harvested at week 2 of tooth extraction showed a large variation in size and granularity as shown in forward and side scatters. The back gating of CD11b+GR1high cells revealed the significant reduction of these cells in ZOL mice. D, dense nucleus small cells (arrowheads) were characteristically associated with TRAP+ osteoclasts (Oc) and necrotic alveolar bone (Nec) in ZOL mice at week 2 of tooth extraction but not found in control (NaCl) mice. Ly6G+ cells in control (NaCl) mice were PMN cells (arrows), whereas Ly6G+ cells in ZOL mice included small cells that did not exhibit a typical PMN cell structure (arrowheads).
Phycoerythrinindotricarbocyanine–Anti Gr1 (Rb6 8c5), supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti-gr1+antibody+clone+1a8/gr1++rb6+8c5/pmc04432939-228-4-6
Average 90 stars, based on 1 article reviews
phycoerythrinindotricarbocyanine–anti-gr1 (rb6-8c5) - by Bioz Stars, 2026-10
90/100 stars
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fat2 antibody / protocadherin fat 2  (NSJ Bioreagents)
99
NSJ Bioreagents fat2 antibody / protocadherin fat 2
Different <t>Ly6G+</t> myeloid cells in the wound healing oral barrier tissue of ZOL mice. A, immunohistology with anti-Ly6G antibody demonstrated clusters of Ly6G+ cells at the epithelial wound margin (not shown) and at the connective tissue interfacing palatal bone (B), both of which were adjacent to the tooth extraction site on day 3. In ZOL mice, fewer Ly6G+ cells were present in both cluster areas. At week 2, ZOL mice exhibited more Ly6G+ cells, in particular adjacent to jawbone oral exposure and/or pustule/fistula (P/F) areas. In some specimens, Ly6G+ cells (arrows) were found around osteoclast (Oc) and in necrotic bone (B). B, the number of Ly6G+ cells in the oral barrier tissue excluding the area of obvious jawbone exposure and P/F areas. *, p < 0.05; n = 4 in each group. C, flow cytometric analysis of the dissociated cells from oral barrier tissue harvested at week 2 of tooth extraction showed a large variation in size and granularity as shown in forward and side scatters. The back gating of CD11b+GR1high cells revealed the significant reduction of these cells in ZOL mice. D, dense nucleus small cells (arrowheads) were characteristically associated with TRAP+ osteoclasts (Oc) and necrotic alveolar bone (Nec) in ZOL mice at week 2 of tooth extraction but not found in control (NaCl) mice. Ly6G+ cells in control (NaCl) mice were PMN cells (arrows), whereas Ly6G+ cells in ZOL mice included small cells that did not exhibit a typical PMN cell structure (arrowheads).
Fat2 Antibody / Protocadherin Fat 2, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti-gr1+antibody+clone+1a8/FAT2+Antibody+%2F+Protocadherin+Fat+2/custom%40v3388%4035175636
Average 99 stars, based on 1 article reviews
fat2 antibody / protocadherin fat 2 - by Bioz Stars, 2026-10
99/100 stars
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Image Search Results


MMP-8 decreases neutrophil infiltration. A: Mean ± SEM of results of gene expression analysis of chemokines (IL-1β) and CXCL1 mRNA transcripts in whole cornea of animals subjected to ocular burn with desiccating stress for 2 or 5 days and topically treated with either Dex or BSS in MMP-8KO or WT mice. ***P < 0.001; ****P < 0.0001 BSS versus UT in WT, BSS versus UT in MMP-8KO. B: Representative merged pictures of IL-1β (green) immunofluorescent staining of central cornea cryosections from animals subjected to a combined model of alkali burn and dry eye topically treated with either Dex or BSS in wild-type (WT) or MMP-8KO mice. Counterstaining was PI = red. C: Representative pictures of Gr-1+ cells (red) of central cornea cryosections from animals subjected to a combined model of alkali burn and dry eye topically treated with either Dex or BSS in wild-type (WT) or MMP-8KO mice (10×). Insets are high magnification of the area below. D: Bar graphs (mean ± SEM) of Gr-1+ cell counts in whole cornea/groups. E: Myeloperoxidase (MPO) activity in whole corneas lysates from corneas subjected to ocular burn with concomitant desiccating stress and topically treated with either Dex or BSS in wild-type (WT) or MMP-8KO mice (mean ± SEM). Dex, Dexamethasone Sodium Phosphate; BSS, Balanced Salt Solution; UT= untreated control; NC=negative control

Journal: Journal of cellular physiology

Article Title: MMP-8 Is Critical for Dexamethasone Therapy in Alkali-Burned Corneas Under Dry Eye Conditions

doi: 10.1002/jcp.25364

Figure Lengend Snippet: MMP-8 decreases neutrophil infiltration. A: Mean ± SEM of results of gene expression analysis of chemokines (IL-1β) and CXCL1 mRNA transcripts in whole cornea of animals subjected to ocular burn with desiccating stress for 2 or 5 days and topically treated with either Dex or BSS in MMP-8KO or WT mice. ***P < 0.001; ****P < 0.0001 BSS versus UT in WT, BSS versus UT in MMP-8KO. B: Representative merged pictures of IL-1β (green) immunofluorescent staining of central cornea cryosections from animals subjected to a combined model of alkali burn and dry eye topically treated with either Dex or BSS in wild-type (WT) or MMP-8KO mice. Counterstaining was PI = red. C: Representative pictures of Gr-1+ cells (red) of central cornea cryosections from animals subjected to a combined model of alkali burn and dry eye topically treated with either Dex or BSS in wild-type (WT) or MMP-8KO mice (10×). Insets are high magnification of the area below. D: Bar graphs (mean ± SEM) of Gr-1+ cell counts in whole cornea/groups. E: Myeloperoxidase (MPO) activity in whole corneas lysates from corneas subjected to ocular burn with concomitant desiccating stress and topically treated with either Dex or BSS in wild-type (WT) or MMP-8KO mice (mean ± SEM). Dex, Dexamethasone Sodium Phosphate; BSS, Balanced Salt Solution; UT= untreated control; NC=negative control

Article Snippet: Immunohistochemistry was performed to detect neutrophils using rat anti-Gr-1 antibody (Ly6G; 1:250, clone 1A8, BD Pharmingen, San Diego, CA).

Techniques: Expressing, Staining, Activity Assay, Negative Control

Different Ly6G+ myeloid cells in the wound healing oral barrier tissue of ZOL mice. A, immunohistology with anti-Ly6G antibody demonstrated clusters of Ly6G+ cells at the epithelial wound margin (not shown) and at the connective tissue interfacing palatal bone (B), both of which were adjacent to the tooth extraction site on day 3. In ZOL mice, fewer Ly6G+ cells were present in both cluster areas. At week 2, ZOL mice exhibited more Ly6G+ cells, in particular adjacent to jawbone oral exposure and/or pustule/fistula (P/F) areas. In some specimens, Ly6G+ cells (arrows) were found around osteoclast (Oc) and in necrotic bone (B). B, the number of Ly6G+ cells in the oral barrier tissue excluding the area of obvious jawbone exposure and P/F areas. *, p < 0.05; n = 4 in each group. C, flow cytometric analysis of the dissociated cells from oral barrier tissue harvested at week 2 of tooth extraction showed a large variation in size and granularity as shown in forward and side scatters. The back gating of CD11b+GR1high cells revealed the significant reduction of these cells in ZOL mice. D, dense nucleus small cells (arrowheads) were characteristically associated with TRAP+ osteoclasts (Oc) and necrotic alveolar bone (Nec) in ZOL mice at week 2 of tooth extraction but not found in control (NaCl) mice. Ly6G+ cells in control (NaCl) mice were PMN cells (arrows), whereas Ly6G+ cells in ZOL mice included small cells that did not exhibit a typical PMN cell structure (arrowheads).

Journal: The Journal of Biological Chemistry

Article Title: Plasticity of Myeloid Cells during Oral Barrier Wound Healing and the Development of Bisphosphonate-related Osteonecrosis of the Jaw *

doi: 10.1074/jbc.M116.735795

Figure Lengend Snippet: Different Ly6G+ myeloid cells in the wound healing oral barrier tissue of ZOL mice. A, immunohistology with anti-Ly6G antibody demonstrated clusters of Ly6G+ cells at the epithelial wound margin (not shown) and at the connective tissue interfacing palatal bone (B), both of which were adjacent to the tooth extraction site on day 3. In ZOL mice, fewer Ly6G+ cells were present in both cluster areas. At week 2, ZOL mice exhibited more Ly6G+ cells, in particular adjacent to jawbone oral exposure and/or pustule/fistula (P/F) areas. In some specimens, Ly6G+ cells (arrows) were found around osteoclast (Oc) and in necrotic bone (B). B, the number of Ly6G+ cells in the oral barrier tissue excluding the area of obvious jawbone exposure and P/F areas. *, p < 0.05; n = 4 in each group. C, flow cytometric analysis of the dissociated cells from oral barrier tissue harvested at week 2 of tooth extraction showed a large variation in size and granularity as shown in forward and side scatters. The back gating of CD11b+GR1high cells revealed the significant reduction of these cells in ZOL mice. D, dense nucleus small cells (arrowheads) were characteristically associated with TRAP+ osteoclasts (Oc) and necrotic alveolar bone (Nec) in ZOL mice at week 2 of tooth extraction but not found in control (NaCl) mice. Ly6G+ cells in control (NaCl) mice were PMN cells (arrows), whereas Ly6G+ cells in ZOL mice included small cells that did not exhibit a typical PMN cell structure (arrowheads).

Article Snippet: To further test the role of Ly6G+/Gr1+ cells on the tooth extraction wound healing, we injected anti-Ly6G (Gr1) antibody (InVivoPlus anti-mouse Ly6G (Gr-1), Bio X Cell, West Lebanon, NH).

Techniques: Extraction, Control

Effect of ZOL on bone marrow myeloid cells. A, 10 days after a bolus IV injection of 500 μg/kg ZOL, the maxillary first molar was extracted, and femur samples were harvested at day 3, week 2, and week 4 of tooth extraction. Reconstructed micro-CT images of the distal epiphyseal trabecular bone indicated the increased bone structure in ZOL mice. B, micro-CT quantitative measurements indicated the significant and progressive increase of femur trabecular bone structure in ZOL mice as depicted by bone volume/tissue volume (BV/TV) and connectivity density (Conn.D). **, p < 0.01; ***, p < 0.001; ****, p < 0.0001; n = 7 in each group. C, the total cell gate flow cytometric analysis of bone marrow flow through cells identified CD11b+ and Ly6G+ cells with various staining intensity during the tooth extraction wound healing period. At week 4, a noticeable decrease of CD11b+Ly6G+ cells in the ZOL group was indicated as compared with the control (NaCl) group. D, the bone marrow cells were proved for CD11b+ and Ly6C+ cells. The variation in staining intensity was observed. In day 3 and week 2, CD11b+Ly6C+ cells appeared to moderately increase in the ZOL group.

Journal: The Journal of Biological Chemistry

Article Title: Plasticity of Myeloid Cells during Oral Barrier Wound Healing and the Development of Bisphosphonate-related Osteonecrosis of the Jaw *

doi: 10.1074/jbc.M116.735795

Figure Lengend Snippet: Effect of ZOL on bone marrow myeloid cells. A, 10 days after a bolus IV injection of 500 μg/kg ZOL, the maxillary first molar was extracted, and femur samples were harvested at day 3, week 2, and week 4 of tooth extraction. Reconstructed micro-CT images of the distal epiphyseal trabecular bone indicated the increased bone structure in ZOL mice. B, micro-CT quantitative measurements indicated the significant and progressive increase of femur trabecular bone structure in ZOL mice as depicted by bone volume/tissue volume (BV/TV) and connectivity density (Conn.D). **, p < 0.01; ***, p < 0.001; ****, p < 0.0001; n = 7 in each group. C, the total cell gate flow cytometric analysis of bone marrow flow through cells identified CD11b+ and Ly6G+ cells with various staining intensity during the tooth extraction wound healing period. At week 4, a noticeable decrease of CD11b+Ly6G+ cells in the ZOL group was indicated as compared with the control (NaCl) group. D, the bone marrow cells were proved for CD11b+ and Ly6C+ cells. The variation in staining intensity was observed. In day 3 and week 2, CD11b+Ly6C+ cells appeared to moderately increase in the ZOL group.

Article Snippet: To further test the role of Ly6G+/Gr1+ cells on the tooth extraction wound healing, we injected anti-Ly6G (Gr1) antibody (InVivoPlus anti-mouse Ly6G (Gr-1), Bio X Cell, West Lebanon, NH).

Techniques: IV Injection, Extraction, Micro-CT, Staining, Control

Effect of anti-Ly6G (Gr1) neutralizing antibody injection on bone marrow and peripheral blood. A, after a bolus IV injection of ZOL (500 μg/kg) or 0.9% NaCl vehicle solution and maxillary molar extraction, daily i.p. injections of anti-Ly6G (Gr1) neutralizing antibody (300 μg/animal/day) were performed starting from day 9 to day 13 of tooth extraction. Bone marrow flow-through cells and peripheral blood samples were harvested 1 day after the last anti-Ly6G (Gr1) antibody injection or on week 2 of tooth extraction. B, flow cytometric analysis of myeloid cell gate of bone marrow flow-through cells indicated that antibody-untreated and isotype control antibody-injected mice presented the high concentration of CD11b+Gr1hi cells or CD11b+Ly6Ghi cells, respectively. By contrast, anti-Ly6G (Gr1) antibody-injected mice contained largely CD11b+Gr1int cells with much reduced presence of CD11b+Gr1hi cells. ***, p < 0.001; n = 3 in each group. C, the myeloid cell gate of peripheral blood samples similarly suggested that the reduction of circulating CD11b+Gr1hi neutrophils in anti-Ly6G (Gr1) antibody-injected mice. ***, p < 0.01; n = 3 in each group.

Journal: The Journal of Biological Chemistry

Article Title: Plasticity of Myeloid Cells during Oral Barrier Wound Healing and the Development of Bisphosphonate-related Osteonecrosis of the Jaw *

doi: 10.1074/jbc.M116.735795

Figure Lengend Snippet: Effect of anti-Ly6G (Gr1) neutralizing antibody injection on bone marrow and peripheral blood. A, after a bolus IV injection of ZOL (500 μg/kg) or 0.9% NaCl vehicle solution and maxillary molar extraction, daily i.p. injections of anti-Ly6G (Gr1) neutralizing antibody (300 μg/animal/day) were performed starting from day 9 to day 13 of tooth extraction. Bone marrow flow-through cells and peripheral blood samples were harvested 1 day after the last anti-Ly6G (Gr1) antibody injection or on week 2 of tooth extraction. B, flow cytometric analysis of myeloid cell gate of bone marrow flow-through cells indicated that antibody-untreated and isotype control antibody-injected mice presented the high concentration of CD11b+Gr1hi cells or CD11b+Ly6Ghi cells, respectively. By contrast, anti-Ly6G (Gr1) antibody-injected mice contained largely CD11b+Gr1int cells with much reduced presence of CD11b+Gr1hi cells. ***, p < 0.001; n = 3 in each group. C, the myeloid cell gate of peripheral blood samples similarly suggested that the reduction of circulating CD11b+Gr1hi neutrophils in anti-Ly6G (Gr1) antibody-injected mice. ***, p < 0.01; n = 3 in each group.

Article Snippet: To further test the role of Ly6G+/Gr1+ cells on the tooth extraction wound healing, we injected anti-Ly6G (Gr1) antibody (InVivoPlus anti-mouse Ly6G (Gr-1), Bio X Cell, West Lebanon, NH).

Techniques: Injection, IV Injection, Extraction, Control, Concentration Assay

Prevention of ONJ-like lesion by anti-Ly6G (Gr1) neutralizing antibody. A, gingival swelling at the tooth extraction site (arrowheads) was exacerbated in ZOL mice, which also exhibited delayed wound closure (arrows). Mice that received isotype control antibody injection showed essentially identical reactions as the antibody-untreated mice. By contrast, anti-Ly6G (Gr1) antibody injections resulted in significantly attenuated gingival swelling in both control (NaCl) and ZOL mice. There was no open wound in anti-Ly6G (Gr1) antibody-injected ZOL mice on week 2 of tooth extraction. B, the area of histological osteonecrosis in the palatal bone was increased in ZOL mice. However, anti-Ly6G (Gr1) antibody injection appeared to attenuate the development of osteonecrosis. **, p < 0.01; n = 6 in each group. C, histological analysis revealed a localized inflammatory reaction (Inf, double arrow) in gingival connective tissue (G.Co.) interfacing palatal bone of control (NaCl) mice with the isotype control antibody injection. ZOL mice with the isotype control antibody injection exhibited not only the localized inflammation but also a large area of diffused inflammatory lesion, which was associated with osteonecrosis (Nec) and epithelial hyperplasia (Epi). Anti-Ly6G (Gr1) antibody injection resulted in the complete remission of inflammatory reaction in the gingival connective tissue interfacing palatal bone of both control (NaCl) and ZOL mice. New bone formation (N.B.) in the tooth extraction socket was noted in the anti-Ly6G (Gr1) antibody injection groups. D, immunohistochemical evaluation showed the presence of Ly6G+ cells in the diffused inflammatory zone (Inf, double arrow) of ZOL mice with isotype control antibody injection. In ZOL mice with anti-Ly6G (Gr1) antibody injection, Ly6G+ cells were not observed in the gingival connective tissue (G.Co) interfacing the palatal bone.

Journal: The Journal of Biological Chemistry

Article Title: Plasticity of Myeloid Cells during Oral Barrier Wound Healing and the Development of Bisphosphonate-related Osteonecrosis of the Jaw *

doi: 10.1074/jbc.M116.735795

Figure Lengend Snippet: Prevention of ONJ-like lesion by anti-Ly6G (Gr1) neutralizing antibody. A, gingival swelling at the tooth extraction site (arrowheads) was exacerbated in ZOL mice, which also exhibited delayed wound closure (arrows). Mice that received isotype control antibody injection showed essentially identical reactions as the antibody-untreated mice. By contrast, anti-Ly6G (Gr1) antibody injections resulted in significantly attenuated gingival swelling in both control (NaCl) and ZOL mice. There was no open wound in anti-Ly6G (Gr1) antibody-injected ZOL mice on week 2 of tooth extraction. B, the area of histological osteonecrosis in the palatal bone was increased in ZOL mice. However, anti-Ly6G (Gr1) antibody injection appeared to attenuate the development of osteonecrosis. **, p < 0.01; n = 6 in each group. C, histological analysis revealed a localized inflammatory reaction (Inf, double arrow) in gingival connective tissue (G.Co.) interfacing palatal bone of control (NaCl) mice with the isotype control antibody injection. ZOL mice with the isotype control antibody injection exhibited not only the localized inflammation but also a large area of diffused inflammatory lesion, which was associated with osteonecrosis (Nec) and epithelial hyperplasia (Epi). Anti-Ly6G (Gr1) antibody injection resulted in the complete remission of inflammatory reaction in the gingival connective tissue interfacing palatal bone of both control (NaCl) and ZOL mice. New bone formation (N.B.) in the tooth extraction socket was noted in the anti-Ly6G (Gr1) antibody injection groups. D, immunohistochemical evaluation showed the presence of Ly6G+ cells in the diffused inflammatory zone (Inf, double arrow) of ZOL mice with isotype control antibody injection. In ZOL mice with anti-Ly6G (Gr1) antibody injection, Ly6G+ cells were not observed in the gingival connective tissue (G.Co) interfacing the palatal bone.

Article Snippet: To further test the role of Ly6G+/Gr1+ cells on the tooth extraction wound healing, we injected anti-Ly6G (Gr1) antibody (InVivoPlus anti-mouse Ly6G (Gr-1), Bio X Cell, West Lebanon, NH).

Techniques: Extraction, Control, Injection, Immunohistochemical staining